rabbit polyclonal anti asc  (MedChemExpress)

Journal: Journal of Neuroinflammation

Article Title: Ethanol consumption aggravates amyloid pathology and neuroinflammation in Alzheimer’s disease associated with inflammasome activation and ASC speck propagation

doi: 10.1186/s12974-025-03501-8

Figure Lengend Snippet: Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, AL177 staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males

Article Snippet: Rabbit polyclonal anti-ASC (AL177) , Adipogen , AG-25B-0006-C100.

Techniques: Western Blot, Activity Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Staining